P. LiWang Protocol Page

SOB

                    Tryptone                         20g
                    Yeast Extrace                   5g
                    NaCl                              0.5g
                    250mM KCl                   10ml

                    Add water to 900ml, adjust to pH 7, add water to 990ml, autoclave, cool to room temp, add 10ml of sterile 1M magnesium chloride before use

Protocols used in the P. LiWang Group School of Natural Sciences University of California Merced
	SOB Tryptone 20g Yeast Extrace 5g NaCl 0.5g 250mM KCl 10ml Add water to 900ml, adjust to pH 7, add water to 990ml, autoclave, cool to room temp, add 10ml of sterile 1M magnesium chloride before use

Protocols used in the P. LiWang Group