P. LiWang Protocol Page

1X Minimal Media

To 900ml autoclaved water add:

                        10X Minimal Salts                                 85 ml
                        Trace Elements Solution                        5ml
                        1M MgCl₂^.6H₂O                                      5ml
                        Thiamine(20mg/ml)                                  2ml
                        Ampicillin(100mg/ml)                               2ml
                        20% Glucose (for ¹²C prep)                    40ml
                        ¹³C glucose (for ¹³C prep)                           3g (in water, sterile-filtered)

Protocols used in the P. LiWang Group School of Natural Sciences University of California Merced
	1X Minimal Media To 900ml autoclaved water add: 10X Minimal Salts 85 ml Trace Elements Solution 5ml 1M MgCl₂^.6H₂O 5ml Thiamine(20mg/ml) 2ml Ampicillin(100mg/ml) 2ml 20% Glucose (for ¹²C prep) 40ml ¹³C glucose (for ¹³C prep) 3g (in water, sterile-filtered)

Protocols used in the P. LiWang Group