Protocols used in the P. LiWang Group

School of Natural Sciences

University of California Merced

  

Luria Broth (LB)

                                   Method 1
                    Tryptone                    10g
                    Yeast Extract               5g
                    NaCl                          10g

                   Water to 1L
                   Adjust pH to 7.0

                   For agar plates add 15g per liter
 

                                   Method 2
                    Add 25g store bought media to 1L water

                    For agar plates add 15g per liter
 
 

Low Salt LB



                    Tryptone                    10g
                    Yeast Extract               5g
                    NaCl                            5g

                    Water to 1L
                   Adjust pH to 7.0

                   For agar plates add 15g per liter