P. LiWang Protocol Page

10X N15 Minimal Salts
(g per 100ml of 10X)

                    KH₂PO₄                                13.0g
                    K₂HPO₄                                10.0g
                       or K₂HPO₄^.3H₂O              13.1g
                    Na₂HPO₄                               9.0g
                    K₂SO₄                                    2.4g
¹⁵NH₄Cl                                   1.1g

Adjust the pH of the solution to 7.20-7.4 using KOH pellets.
The pH before adjustment should be around 6.7

Protocols used in the P. LiWang Group School of Natural Sciences University of California Merced
	10X N15 Minimal Salts *(g per 100ml of 10X)* KH₂PO₄ 13.0g K₂HPO₄ 10.0g or K₂HPO₄^.3H₂O 13.1g Na₂HPO₄ 9.0g K₂SO₄ 2.4g ¹⁵NH₄Cl 1.1g Adjust the pH of the solution to 7.20-7.4 using KOH pellets. The pH before adjustment should be around 6.7

Protocols used in the P. LiWang Group